Wham is designed for paired-stop Illumina libraries with regular insert dimensions (~300bp-500bp). Wham integrates mate-pair mapping, split study mapping, delicate-clipping, substitute alignment and consensus sequence centered evidence to forecast SV breakpoints with single-nucleotide accuracy. Wham generates a combined pileup (catalog of reads covering a placement in the genome) for all BAM information provided. Reads from all people today included in joint contacting which can be gentle or really hard clipped are hashed by placement to establish shared breakpoints. Positions during the pileup where by three or maybe more primary reads share the same breakpoint are interrogated as being a putative SV.
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Wham So fills the need for a fast, user friendly SV caller and association-testing Resource which is compatible with most common variant contacting pipelines.
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Through joint calling at least one particular person have to have three reads supporting the alternative allele. This filter prevents randomly shared begin and stop comfortable clipping across people from triggering a non-reference allele connect with.
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